Beyond DAPI: Cleaner, Sharper Counterstaining with NucSpot® Nuclear Stains

PFA-fixed, Triton® X-100 permeabilized HeLa cells stained with NucSpot® Nuclear Stains in PBS and imaged by confocal microscopy without a wash step. All stains were used at 1X concentration except for NucSpot® 750/780, which was used at 5X concentration in order to image using 640 nm excitation.
Biotium

NucSpot® Nuclear Stains from Biotium are a family of versatile fluorescent DNA counterstains…